Figure 3
From: Use of folding modulators to improve heterologous protein production in Escherichia coli
Strategy for selection of molecular chaperones and folding catalysts for co-production analyses. Following production of a recombinant protein in E. coli, analysis of cell growth, protein solubility and subcellular location, macromolecular state and activity provide some insight into the limiting step in the folding and production process. This Figure shows the major bottlenecks typically encountered (in hexagons) during production of a difficult-to-express recombinant target and identifies the co-production strategies that have been most successful in overcoming these bottlenecks to date (corresponding ovals).