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Figure 3 | Microbial Cell Factories

Figure 3

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 3

Determination of the different oxygen transfer rates (OTR) in RAMOS. (A) for the chemical 0.5 mM sulfite system; (B) for the E. coli fermentation in WR medium; (C) for the H. polymorpha fermentation in SYN6-MES medium. Operation conditions: 10 mL, 15 mL and 20 mL filling volume, 250 mL Erlenmeyer shake flask for RAMOS, 250 rpm shaking frequency, 50 mm shaking diameter, at 25°C temperature with the sulfite system and 30°C temperature with the microorganisms. The E. coli cultures were induced at 7.17 h with 0.5 mM IPTG.

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Microbial Cell Factories

ISSN: 1475-2859

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