Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Table 5 Measured substrates and products consumed/produced at steady state in each experimental condition

	Fab-expressing strain						Control strain
mmol/gDW·h	21%		11%		8%		21%		11%		8%
		sd		sd		sd		sd		sd		sd
Glucose	-0.99	0.05	-1.33	0.07	-1.74	0.09	-0.99	0.05	-1.32	0.07	-1.92	0.10
OUR	-2.12	0.17	-1.57	0.08	-0.54	0.10	-2.12	0.17	-1.66	0.33	-0.28	0.01
CER	2.37	0.19	2.03	0.10	1.65	0.08	2.37	0.19	2.09	0.42	1.35	0.27
Biomass	3.01	0.30	3.71	0.37	3.73	0.19	3.59	0.36	3.70	0.37	3.76	0.38
Ethanol	-	-	0.33	0.02	1.00	0.05	-	-	0.31	0.02	1.16	0.06
Glycerol	-	-	0.01	0.001	0.02	0.001	-	-	0.02	0.001	0.02	0.001
Citrate	0.03	0.002	0.02	0.001	0.04	0.002	0.01	0.001	0.02	0.001	0.05	0.02
Arabinitol	-	-	0.21	0.01	0.42	0.02	-	-	0.10	0.01	0.40	0.13
Pyruvate	-	-	0.06	0.003	0.09	0.005	-	-	0.06	0.002	0.12	0.01
% C Balance Error excluding arabinitol	6.4%		15.7%		23.9%		1.3%		14.5%		29.3%
% C Balance Error including arabinitol	6.4%		2.5%		3.7%		1.3%		8.2%		11.9%

Substrate consumption, biomass and metabolites production rates, for each strain and the different experimental conditions (% O₂ in the inlet air: 21, 11, 8). Bottom rows: values of the carbon balance mismatch in percentage including or excluding arabinitol. OUR, oxygen uptake rate; CER, CO₂ exchange rate.

ISSN: 1475-2859