Figure 2From: Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regenerationThe wild-type 6pgdh sequence, the deduced amino acid sequence, and the codon-optimized 6pgdh sequence. WT, wt6pgdh; CO, co6pgdh. The highlighted codons among the wt6pgdh gene are the rare codons in E. coli. The codon clusters in blue are the rare codons sites for site-directed mutagenesis. The underlined nucleotides in co6pgdh are the changed ones corresponding to wt6pgdh.Back to article page