Table 1 Primers for qPCR method for determination of PCN in bacterium Escherichia coli containing plasmid pET3a

plasmid

5'-CGGTTGCTGGCGCCTAT-3'

703–721 pET 3a, Novagen

17 bp

83°C

5'-ACCATACCCACGCCGAAA-3'

780–797 pET 3a, Novagen

18 bp

chromosome

5'-GCGAGCGATCCAGAAGATCT-3'

4044875 – 4044894 E. coli K12, Accession number U00096

20 bp

75°C

5'-GGGTAAAGGATGCCACAGACA-3'

4044941–4044961 E. coli K12, Accession number U00096

21 bp