Figure 2From: Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processesInfluence of different sample treatments on Ct values of plasmids and chromosomes in culture of Escherichia coli BL21 (DE3) containing pET3a plasmid with gene for hG-CSF from bioprocess in GYSP medium. Sample treatments are marked as N nontreated, C centrifuged, F frozen, 95 and 99 temperature treatment in °C and are applied in subsequent steps as preformed. Duration of heating for 10 min, 15 min, 20 min at 95°C and 99°C are not presented separately.Back to article page