Table 1 Saccharomyces cerevisiae strains and oligonucleotides used in this study
From: Switching the mode of sucrose utilization by Saccharomyces cerevisiae
| Â | Relevant genotype or description: | Source or reference: |
|---|---|---|
Yeast Strains | Â | Â |
1403-7A | MAT a MAL4 C MGL3 suc - gal3 gal4 trp1 ura3 | ATTC 208023 |
LCM001 | agt1 Δ::kanMX6 derivative of 1403-7A | This study |
S288C | MAT αAGT1 MAL12 mal13 MAL31 MAL32 mal33 gal2 mel flo1 flo8-1 hap1 SUC2 | (39) |
MC966A | MAT a MAL2 ura3-52 his3-11,15 leu2-3,112 SUC2 | (43) |
Primers | Â | Â |
AGT1-pFA6-F1 | AAGCAAGAAGAAGGCTGCCTCAAAAAATGAGGATAAAAACATTTCTGAGCGGATCCCCGGGTTAATTAA | Invitrogen |
AGT1-pFA6-R1 | AAAGGGATTCCTTATTTCTTCCAAAAAAAAAAAAACAACCCTTTTACTTAGAATTCGAGCTCGTTTAAAC | " |
AGT1-F | AGGAGCTCATGAAAAATATCATTTCATTGG | Gibco BRL |
AGT1-R | TTGGATCCACATTTATCAGCT GC | " |
MALx1-F | CCATACTTGTTGTGAGTGG | " |
MALx1-R | TCATTTGTTCACAACAGATG | Invitrogen |
SUC2-F | GCGATAGACCTTTGGTCCAC | Gibco BRL |
SUC2-R | GGACCGTGGTAACTCTAAGG | " |
V-AGT1-F | GAATTTTCGGGTTGGTG | " |
V-AGT1-R | TTGGATCCACATTTATCAGCTGC | " |
V-kanr-R | GGAATCGAATGCAACCGG | " |