Figure 2

Detection of active sucrose transporter genes in yeast strains. Separation of chromosomes of yeast strains by PFGE and staining with ethidium bromide (A), and detection of sucrose transporter genes after chromosomes were blotted onto a nylon membrane and hybridized with a probe for AGT1 (B) or MALx1 (C). Lanes 1, strain 1403-7A; lanes 2, the agt1 Δ strain LCM001. The values on the right in panel A are the sizes (in kilobase pairs) of selected chromosomes, the Roman numerals between panels A and B are chromosome numbers, and the positions of chromosomes carrying AGT1, MAL21, MAL31 and MAL41 are indicated on the right of panel C. Both chromosome sizes and numbers, and locations of transporter genes, were based on results obtained with the reference strains S288C and MC966A (see Table 1, data not shown).