Figure 4
Cultivations with EnBase of E. coli BL21(DE3) in 96-well plates. (a) Effect of different amounts of glucoamylase on the growth. (b) Effect of extra addition of ammonia. Cultivations were performed at 37°C with 150 μl liquid medium per well. Different amounts of glucoamylase, as indicated in the figure, were added at the time of inoculation in (a). (b) All cultivations were performed with an initial concentration of 6 AGU l-1 and extra ammonia (5 μl of a 1% stock solution) was added after 20 h of cultivation (arrow) providing a calculated extra ammonia concentration of 0.29 g ammonia per l liquid medium. The cell density (OD600) was analyzed from 5 μL samples in all cases. The error bars reflect cultivations in 5 parallel wells of one plate (a) and 10 parallel wells per plate (b). Evaporation per well was 0.125 μl per h-1.