Experiment type | Analyses to perform for evaluating proteins before starting the experiments | Protocol references (from a list of accepted standard protocols for each experiment type that guarantee output congruency) | Equipments (define minimal requisites of the devices in terms of quality, maintenance, controls) |
---|---|---|---|
Protein pull-down | SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; FT-IR; NMR; | SDS: protocol 1 (or protocol 2,..) AI protocol 1 | SDS: Hoefer minigel.... AI: Spectrofluorimeter Jasco ..... SEC: column xy and FPLC model z..... |
Monoclonal Ab purification | SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; | Â | Â |
Protein purification | SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; FT-IR; NMR; | Â | Â |
SPR | Â | Â | Â |
ITC | Â | Â | Â |
ELISA | Â | Â | Â |
Enzymatic assay | Â | Â | Â |
Protein refolding | Â | Â | Â |
Immunoprecipitation | Â | Â | Â |
Immunofluorescence | Â | Â | Â |
Immunohistochemistry | Â | Â | Â |
Proximity ligation | Â | Â | Â |