Quantifying the metabolic capabilities of engineered Zymomonas mobilis using linear programming analysis

Table 5 Number of double deletions leading to x% decrease in the maximum ethanol or growth yield

			x = 0	0 < x < 50		50 ≤ x < 100			x = 100
		Single Deletions	x = 0	x = 0	0 < x < 50	x = 0	0 < x < 50	50 ≤ x < 100	x = 0	0 < x < 50	50 ≤ x < 100
Ethanol Yield	Glc	x = 0	231	0	154	0	0	173	0	0	3
		0 < x < 50	N/A		21		0	35		0	21
		50 ≤ x < 100	N/A	N/A	N/A			28			0
	Xyl	x = 0	231	0	44	0	0	142	0	0	12
		0 < x < 50	N/A		0		0	5		1	9
		50 ≤ x < 100	N/A	N/A	N/A			11			10
Growth Yield	Glc	x = 0	91	0	186	0	1		0	9
		0 < x < 50	N/A		68		1			22
	Xyl	x = 0	133	2	117	0	7		1	12
		0 < x < 50	N/A		20		0			8

Both glucose and xylose substrate cases are depicted. The category of the single deletion of the genes involved in the double deletions with respect to their impact on the objective value (see Table 4) is also depicted.

ISSN: 1475-2859