Figure 4

Purification and analysis of anti-CRP LA13-IIE3 scFvs. A Westernblot and immunostain of the combined supernatant of 3 independent productions, wash fractions and elution fractions of the IMAC purification. Samples were separated by reducing SDS-PAGE (12%) and detected as described in figure 3. B Antigen binding ELISA of purified anti-CRP scFv produced in B. megaterium or E. coli. Antigens: 100 ng/well CRP or 100 ng/well control protein BSA, detection as described in figure 2.