Figure 2

Analysis of functional anti-lysozyme D1.3 scFab after 0, 6 and 24 h of production. A Ammonium sulfate precipated scFabs from 0.75 mL supernatant were separated by reducing SDS-PAGE (10%) and detected using mAb mouse anti-His and goat anti-mouse IgG AP (Fc specific). B Antigen binding ELISA with 50 μL culture supernatant from a production in 100 mL scale. Mean values and standard deviations of data obtained from three different experiments are given. Antigens: 1 μg/well lysozyme or 1 μg/well control protein BSA. The D1.3 scFabs were detected using mAb mouse anti-His and goat anti-mouse IgG HRP (Fc specific).