Figure 1
From: Production of single chain Fab (scFab) fragments in Bacillus megaterium
Plasmid map of pEJBmD1.3scFab. Abbreviations: bla: β-lactamase gene for ampicillin resistance; colE1: E. coli origin of plasmid replication; His-tag: synthetic 6xhistidine tag; ori: B. megaterium origin of plasmid replication; P xylA : xylose inducible promoter; RBS: ribosome binding site; repU: a gene for plasmid replication in B. megaterium; scFab: single chain fragment antigen binding; SPlipA: signal peptide sequence of B. megaterium extracellular esterase LipA; terminator: sequence terminating transcription; tet: tetracyclin resistence gene; LC: light chain; Fd: VH and CH1 of the heavy chain; xylR: xylose repressor