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Table 3 Kinetic parameters of wild-type and mutant PsXRs for NADH and NADPH-dependent reactions

From: Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

   

NADH

NADPH

Enzymes

K m for xylose (NADH) [mM]

K m for xylose (NADPH) [mM]

K m [mM]

kcat [Min-1]

kcat/K m [mM-1·min-1]

K m [mM]

kcat [Min-1]

kcat/K m [mM-1·min-1]

XR

42[a]

42[a]

0.021[a]

NA[b]

NA[b]

0.009[a]

NA[b]

167000[c]

Wild-type NT-XR

90 ± 7

82 ± 4

0.0106 ± 0.0006

923 ± 35

87630 ± 6730

0.0062 ± 0.0014

1650 ± 197

278900 ± 56300

1-2F7

314 ± 17

334 ± 56

0.512 ± 0.022

1110 ± 78

2180 ± 151

0.849 ± 0.045

2110 ± 236

2500 ± 300

2-2C12

291 ± 14

168 ± 22

0.147 ± 0.012

720 ± 57

4900 ± 315

0.427 ± 0.017

158 ± 9

370 ± 19

NT-K270R/N272D

128 ± 8

267 ± 31

0.058 ± 0.009

1075 ± 124

18800 ± 2400

0.142 ± 0.031

955 ± 203

6412 ± 1489

  1. [a] Data were quoted from C. V. K. Verduyn et al [32]. [b] NA, not available. [c] Data were quoted from R. Woodyer et al [22].
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Microbial Cell Factories

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