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Table 2 Specific activity of wild-type and mutant PsXRs in this study.

From: Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

 

Specific activity [U·mg-1]

 

Enzymes

NADH

NADPH

NADH-/NADPH-linked activity ratio

XR

16.7[a]

23.2[a]

0.72

Wild-type NT-XR

18.9 ± 1.3

44.1 ± 2.4

0.43

1-2F7

10.4 ± 0.4

10.8 ± 1.3

0.96

2-2C12

20.6 ± 2.3

0.82 ± 0.16

25.2

NT-K270R/N272D

19.3 ± 1.4

18.1 ± 1.7

1.07

  1. [a] Data were quoted from C. V. K. Verduyn et al [32].
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Microbial Cell Factories

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