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Figure 1 | Microbial Cell Factories

Figure 1

From: Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

Figure 1

SDS-PAGE analysis of crude extracts of XR, NT-XR and CT-XR. Expression was carried out in E. coli cells in LB medium containing kanamycin (50 μg ml-1) and IPTG (1 mM). Crude extracts of different variants were adjusted to the same OD600 concentration (12.41 OD/ml) before they were prepared, and 15 μl was loaded in each lane. Lanes: 1, the insoluble fraction of NT-XR; 2, the soluble fraction of NT-XR; 3, the insoluble fraction of pET30a; 4, the soluble fraction of pET30a; 5, marker; 6, the insoluble fraction of XR; 7, the soluble fraction of XR; 8, the soluble fraction of CT-XR; 9, the insoluble fraction of CT-XR.

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Microbial Cell Factories

ISSN: 1475-2859

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