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Table 1 Activity of r-α1-PI from E. coli and A. niger and evaluation of molecular weight of recombinant and plasma-derived species determined by MALDI-MS

From: Expression of human α1-proteinase inhibitor in Aspergillus niger

Protein

Activitya (%)

Molecular massb

pd-α1-PI (standard)

100

50,300

degly-pd-α1-PI

n.a.c

44,210

r-α1-PI/E. coli

35d

45,000e

r-α1-PI/A. niger

76f,g

50,100

  1. aThe calculations were based on comparison with pdtαxtPI as a standard (100%) and using normalized equal concentrations of r-α1-PI samples as determined by ELISA; bmolecular weight is shown as a an average value measured for 2 samples; c not available; pd-α1-PI was enzymatically deglycosylated under denaturing conditions; d as measured for His-tagged r-α1-PI within 1.5 h after elution from the TALON beads; e as estimated by amino acid sequence for α1-PI without glycans; f shown for A. niger supernatant after Amicon 10 K filtration procedure; g the standard deviation of our potency assay is ± 15%.
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Microbial Cell Factories

ISSN: 1475-2859

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