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Table 1 Accumulation of aromatic intermediates, acetate, L-Phe yields and productivities in L-Phe overproducing strains assessed in resting cell cultures containing 1 g of biomass. The results of the JM101-ev2, PB12-ev2 and PB13-ev2 strains have been previously reported [15, 69].

From: Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine

Strains

qGlc (g/gDCW·h)

qAcetate (mg/gDCW·h)

DHS (g/L)

SHIK (g/L)

L-Phe (g/L)

Y Phe/Glc

qPhe (mg/gDCW·h)

JM101-ev2

0.17 ± 0.01

70 ± 0.41

0.020 ± 0.001

0.029 ± 0.002

0.396 ± 0.025

0.22 ± 0.01

36.34 ± 2.50

PB12-ev2

0.12 ± 0.01

30 ± 0.22

0.054 ± 0.003

0.034 ± 0.001

0.460 ± 0.015

0.33 ± 0.01

40.60 ± 3.17

PB13-ev2

0.14 ± 0.01

40 ± 0.25

0.054 ± 0.004

0.034 ± 0.002

0.300 ± 0.019

0.20 ± 0.02

29.25 ± 2.50

  1. Symbols: qGlc, specific consumption rate of glucose; qAcetate, specific consumption rate of acetate; qPhe, specific production rate of phenylalanine; YPhe/Glc, yield of phenylalanine synthesized from glucose. DHS, 5-dehydroshikimate; SHIK, shikimate; gDCW, grams of dry cell weight.