Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

Table 1 ATP leakage (%) of different samples from different cultures.

Samples from same culture				Samples from different cultures
Samples	MeOH/HEPES	Samples	MeOH	Samples	MeOH/HEPES
1	2.3	5	13.0	7	2.5
2	1.6	6	12.0	8	6.4
3	3.7	-	-	9	4.3
Average	2.5 ± 0.9	Average	12.5 ± 0.5	Average	4.4 ± 1.5

Figures in the table indicate the percentage of ATP that was measured in the supernatant of L. plantarum cells after quenching. Samples 1, 2 and 3 come from the same batch culture and were quenched with 70 mM of HEPES (pH 5.5). Samples 7, 8 and 9 come from different batch cultures and were also quenched with MeOH/HEPES solution. Samples 5 and 6 come from the same batch culture but were quenched with 60% aqueous methanol solution. Cultures were grown on CDM with 333 mM and 100 mM of glucose respectively. The percentage was calculated by dividing the amount of ATP in the supernatant by the sum of the amounts of ATP in the supernatants and cell extract.

ISSN: 1475-2859