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Table 2 Specific activities of phosphofructokinase, pyruvate kinase and lactate dehydrogenase in cells (exponential phase) growing in microaerobic glucostat cultures with varying glucose concentration levels

From: Glycolysis and the regulation of glucose transport in Lactococcus lactis spp. lactis in batch and fed-batch culture

 

Sp. Act. of las enzyme a

Glucose concentration (mM)

Phosphofructokinase U/OD600

Pyruvate kinase U/OD600

Lactate dehydrogenase U/OD600

13.75

5.00 ± 0.05

  

27.50

5.40 ± 0.06

12.52 ± 0.07

21.30 ± 0.08

55.00

7.10 ± 0.05 (4.56)b

13.10 ± 0.05 (11.52)b

23.72 ± 0.05 (21.30)b

138.00

6.00 ± 0.04

11.00 ± 0.08

23.50 ± 0.05

277.00

4.00 ± 0.04

8.72 ± 0.05

11.43 ± 0.07

415.00

3.00 ± 0.05

5.61 ± 0.05

10.70 ± 0.09

555.00

2.11 ± 0.05

5.60 ± 0.07

9.81 ± 0.05

  1. aSpecific activities (U/OD600) are micromoles per minute per cell density. Values are averages of at least three independent measurements.
  2. bValues obtained in anaerobic batch cultures with 55 mM initial glucose concentration.