Table 3 Glycation of antibody produced in the first, second, and third series of 2L bioreactor experiments. Each condition was evaluated in duplicate bioreactors. Data represents average ± standard deviation obtained from duplicate cultures.

1

2

Control

19 ± 2 %

2.9 ± 1.1 g/L

1

191

Control

17 ± 2 %

1.9 ± 0.4 g/L

1

274

Control

15 ± 1 %

1.4 ± 0.5 g/L

2

2

Control

16 ± 2%

1.8 ± 0.1 g/L

2

2

Partial Continuous Glucose Feed

10 ± 1%

0.8 ± 0.1 g/L

3

2

Control

19 ± 1%

2.2 ± 0.2 g/L

3

2

Full Continuous Glucose Feed

6 ± 0 %

0.5 ± 0.4 g/L