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Table 1 Growth parameters of E. coli strains BL21, BL21/pOri1 and BL21/pOri2 cultured in the chemostat system at different growth rates.

From: Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism

Parametera

Value

 

BL21

BL21/pOri1

BL21/pOri2

QGluc

10.96

35.00

36.01

μ

0.46

0.39

0.29

qGluc

5.04

13.65

10.46

Ybiomass

0.97

1.95

1.66

ybiomass

0.45

0.76

0.48

rGluc

5.2

7.0

6.3

rAce

0

4.1

4.4

rO2

11.0

16.9

16.5

rCO2

12.2

17.9

17.3

Plas. copies

0

50

410

  1. a The following growth rates were determined when bacteria were cultured for 16 h in the batch cultivation: BL21, 0.46 h-1; BL21/pOri1, 0.39 h-1; BL21/pOri2, 0.29 h-1. Abbreviations: QGluc (mmol), Glucose consumption amount; μ (h-1), growth rate; qGluc (mmol h-1), Glucose consumption rate; Ybiomass (g), Biomass yield; ybiomass (g h-1), Biomass yield rate; rGluc (mmol g-1 h-1), Glucose consumption rate per biomass; rAce (mmol g-1 h-1), Acetate yield per biomass; rO2 (mmol g-1 h-1), Oxygen uptake rates per biomass; rCO2 (mmol g-1 h-1), CO2 secretion per biomass; Plas. copies, Plasmid copy number