Table 1 S. cerevisiae strains and plasmids used in this study.
S. cerevisiae strains | Relevant genotype/phenotype | Reference |
|---|---|---|
JBY25-4M | MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC2, unknown beneficial mutations for arabinose utilization, with plasmids YEparaA, YEparaBG361A, YEparaD, YEpGAL2 | [11] |
BWY02.X | MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC2, unknown beneficial mutations for arabinose utilization, HIS3::YIpXR/XDH/XK | This work |
BWY02.Xp | BWY02.X with plasmids p424H7, p425H7, p426H7 | This work |
BWY02.XA | BWY02.X with plasmids YEpURAaraA, YEparaBG361A, YEparaD | This work |
TMB 3400 | HIS3::pPGK-Xyl1-tPGK, pADH-Xyl2-tADH, pPGK-XKS1-tPGK | [18] |
TMB 3060 | TMB 3400, TRP1::YIpAraB KanMX | This work |
TMB 3061 | TMB 3060, NTS2::pHXT7-AraA-tCYC1 and NTS2::pHXT7-AraD-tCYC1, KanMX | This work |
TMB 3063 | TMB 3061 retransformed with NTS2::pHXT7-AraA-tCYC1 | This work |
CEN.PK 113-7D | MATa (prototrophic) | [16] |
Plasmids | ||
YIpXR/XDH/XK | pPGK1-XYL1-tPGK1, pADH1-XYL2-tADH1, pPGK1-XKS1-tPGK1, HIS3 | [7] |
YEparaA | pHXT7-AraA-tCYC1, HIS3 | [11] |
YEpURAaraA | pHXT7-AraA-tCYC, URA3 | This work |
YEparaB | pHXT7-AraB-tCYC1, TRP1 | [11] |
YEparaD | pHXT7-AraD-tCYC1, LEU2 | [11] |
p423H7 | pHXT7-MCS-tCYC1, HIS3 | [11] |
p424H7 | pHXT7-MCS-tCYC1, TRP1 | [11] |
p425H7 | pHXT7-MCS-tCYC1,, LEU2 | [11] |
p426H7 | pHXT7-MCS-tCYC1, URA3 | [11] |
pBluescript II SK | Cloning vector | (Stratagene, La Jolla, CA, USA) |
YIpAraB | KanMX, pHXT7-AraB-tCYC1 TRP1 | This work |
prDNA | pBluescript, NTS2 | This work |
prDNAAraA | prDNA, NTS2::pHXT7-AraA-tCYC1 | This work |
prDNAAraD | prDNA, NTS2::pHXT7-AraD-tCYC1 | This work |