Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Table 6 PGAP promoter cultures comparison

Protein	Promoter		Substrate	Total protein (mg/L)¹ (U/mL)²	Productivity (mg/L h)¹ (U/L h)²	Operational mode	Bioreactor	Reference
Insect esterase	GAP	Intracellular	Glucose	7¹	--	Batch	Shake flasks 50 mL	[94]
		Extracellular		80¹	--
	AOX1	Extracellular	Methanol	40¹	--
Fructose exo-levanase	GAP	Extracellular	Glycerol	26.6²	682²	Fed-batch	Bioreactor 7.5 L	[20]
	AOX1	Extracellular	Methanol	21.1²	220²	Fed-batch
Human trysinogen	GAP	Extracellular	Glycerol	--	--	Fed-batch	Bioreactor 2 L	[21]
	AOX1	Extracellular	Methanol	--	--	Fed-batch
Vitellogenin	GAP	Intracellular	Glucose	12²	--	Fed-batch	Bioreactor 2 L	[98]
hGM-CSF	GAP	Extracellular	Glucose	90¹	1.2¹	Batch	Shake flasks 50 mL	[93]
	GAP+AOX1	Extracellular	Methanol	180¹	2.4¹	Batch
Aqualysin I	GAP	Extracellular	Glucose	1000¹		Batch	Shake flasks	[100]
h-Chitinase	GAP	Extracellular	Glucose	450¹	2.8¹	Fed-batch	Bioreactor 3 L	[84]
				--	15¹	Continuous
	AOX	Extracellular	Methanol	350¹	2.9¹	Fed-batch
HBsAg	GAP	Extracellular	Glucose	--	--	Fed-batch	Shake flasks 500 mL	[96]
	GAP multicopy	Extracellular		--	--	Fed-batch
				--	--	Cyclic batch
	AOX1	Extracellular	Methanol	--	--	Fed-batch
Cellobiohydrolase	GAP	Extracellular	Glycerol	--	--	Fed-batch	Bioreactor 7.5 L	[92]
	AOX1	Extracellular	Methanol	--	--	Fed-batch

ISSN: 1475-2859