Table 2 Plasmids and genes used to construct vectors of the pT7RS derivatives
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Vectors | Gene cloned | Result ing vectors | Expression products | Name of the protein used in the text |
|---|---|---|---|---|
pT7SR | Ubiquitin | pT7U | Ubiquitin | UBI |
pT7U | K | pT7UK | UBI::K | UBI::K |
| Â | UBP1 | pT7UP | UBI::UBP1 | UBP1 |
pT7CH | UBI::UBP1 with Q754L mutation | pT7UPQL | UBI::UBPQL::6xHis | UBPQL |
| Â | UBI::UBP1(55-809) without Q754L mutation | pT7UPD | UBI::UBPD::6xHis | UBPD |
| Â | UBI::UBP1(55-809) with Q754L mutation | pT7UPDQL | UBI::UBPDQL::6xHis | UBPDQL |
pT7CH | UBI::UBP1(99-809) with Q754L mutation | pT7UPD2QL | UBI::UBPD2QL::6xHis | UBPD2QL |
pT7NH | Ubiquitin | pT7NHU | 6xHis::UBI | 6xHisUBI |
pT7NHU | S | pT7NHUS | 6xHis::UBI::S | 6xHisUBI::S |