Figure 2From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coliDependence of the expression level of the shorter analogue of UBP1, UBP1D2QL, on the time after IPTG induction as revealed by SDS electrophoresis in 12% polyacrylamide gel. E. coli BL21 (DE3) cells transformed by the plasmid pT7UPD2QL were cultured at 25°C in LB, and induced by the addition of IPTG to the final concentration of 1 mM. Aliquots of cultures were taken at: 1 – 0 min, 2 – 30 min, 3 – 60 min, 4 – 90 min, 5 – 120 min, 6 – 150 min after induction.Back to article page