Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: The case of lysostaphin

Table 1 Strains and Plasmids

Bacterial strain/plasmid	Properties	Reference
*Bacteria*
Lactococcus lactis subsp. cremoris NZ3900	Integration of nisRnisK in the chromosome; integration of the lac operon in the in the chromosome and deletion of lacF resulting in a lactose- negative host strain that can be complemented by lacF	[25]
*Plasmids*
pNZ8148	P_nisA, Cm^R; replicon of rolling circle plasmid pSH71, basic NICE vector, derivative of pNZ8048	[12] [26]
pNZ1709	Lysostaphin gene under control of P_nisA, Cm^R; derivative of pNZ8148	This work
pNZ1710	Lysostaphin gene under control of P_nisA, lacF; expression vector for food- grade expression, selection on growth with lactose; derivative of pNZ1709	This work [11]

ISSN: 1475-2859