Figure 8From: Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor culturesA: Western blot analysis of samples from the MLFB run at pH 5.0 and 30°C. B: Western blot analysis of samples from the TLFB. C: Western blot analysis of samples from the TLFB with an early induction. Antibodies directed against the CALB moiety were used. CBM-CALB appears as diffuse bands in the range 39–44 kDa due to glycosylation [16].Back to article page