Figure 5From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysatesLevel of 18S rRNA of S. cerevisiae carlsbergensis determined by sandwich hybridization as molecules per cell (●) in different growth states. The growth was followed by measuring the optical density at 600 nm (○) and the specific growth rate μ (□) was determined. The yeast was cultured in medium containing 1% yeast extract, 2% Bacto peptone solution and 2% glucose as carbon source. 1 × 106 – 3 × 106 yeast cells as crude lysate was added to sandwich hybridization for 18S rRNA analysis. OD600 = 1 corresponds to 5.5 × 108yeast cells ml-1. The error bars of show the ± SD of three parallel samples.Back to article page