Recombinant S. cerevisiae expressing Old Yellow Enzymes from non-conventional yeasts: an easy system for selective reduction of activated alkenes

Table 2 Bioreductions of alkyl-substituted cyclohex-2-enones (substrates 4a, 5a, 6a, and 7a ^# ) with recombinant S. cerevisiae BY4741 ΔOye2 bearing heterologous OYE genes

OYE gene source	4a → 4b		5a → 5b		6a → 6b^‡
	Time	Yield	Time	Yield	Time	Yield	(S)-6b
	h	%	h	%	h	%	e.e.%
C. castellii DBVPG3704	2	≥ 99	2	76^†	72	< 5	n.d.
C. sake DBVPG6162	1	≥ 99	1	95	48	75	≥ 98
K. exigua L10	2	≥ 99	2	92	72	23	≥ 98
K. lodderae DBVPG6308	2	≥ 99	2	84^†	72	25	≥ 98
K. spencerorum DBVPG6748	2	≥ 99	1	95	48	60	≥ 98
Nak. bacillisporus DBVPG6945	1	≥ 99	2	94	72	24	≥ 98
Nau. castellii DBVPG6298	2	≥ 99	2	95	48	53	≥ 98
S. cerevisiae BY4741 (Oye2)	1	≥ 99	2	96	48	45	≥ 98
S. cerevisiae L12	2	≥ 99	1	97	48	40	≥ 98

Data are reported as molar conversion (%). ^#No conversion of compound 7a was observed in the first 72 h. ^†15-20% of compound 5d was also observed; ^‡product 6c was always less than 15%. The experiment were performed in triplicate, the yield standard deviations (SD) were always less than 4%, and the ee% SD were always less than 3%.

ISSN: 1475-2859