Primer | Sequence and purposea |
---|---|
LP1 | 5’-ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACAGCGATTGTGTAGGCTGGAG-3’, deletion of aroL |
LP2 | 5’-TCAACAATTGATCGTCTGTGCCAGGGCGCTGCGAATTTCAGAAATCACCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL |
P1 | 5’-GTTTCGTTGGCATCGTTCTT-3’, Diagnostic PCR for the deletion of aroL |
P2 | 5’-ATTCTCATGACACCGGCTTT-3’, Diagnostic PCR for the deletion of aroL |
KP1 | 5’-ATGGCAGAGAAACGCAATATCTTTCTGGTTGGGCCTATGGGTGCCGGAAAAGCGATTGTGTAGGCTGGAG-3’, deletion of aroK |
KP2 | 5’-TTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL |
P3 | 5’-GCGAAGCGGGTTTATCATTA-3’, Diagnostic PCR for the deletion of aroK |
P4 | 5’-GTTCCCCGAGAGTAACGAC-3’, Diagnostic PCR for the deletion of aroK |
GP1 | 5’-GCCTGCAGAGGAGGGCGTAAATATGAATTATCAGAACGACGATT-3’, Pst I, PCR for aroG |
GP2 | 5’-CGGCATGCTTACCCGCGACGCGCTTTTACT-3’, Sph I, PCR for aroG |
G15P1 | 5’-CTCAATATGATCACCCCACAAT-3’, sited-specific mutagenesis of aroG |
G15P2 | 5’-AAACTCACCTGCCGCTGGCAGACCG-3’, sited-specific mutagenesis of aroG |
EP1 | 5’-GCGAATTCAGGAGG TAATAAATATGGAAACCTAT GCTGTTTT-3’, Eco RI, PCR for aroE |
EP2 | 5’-CGGCGGCCGCTTAT CACGCGGACAATTCCTCCT-3’, Not I, PCR for aroE |
BP1 | 5’-CGGCGGCCGCAGGAGG TAATAAATATGGAGAGGATTGTCGTTAC-3’, Not I, PCR for aroB |
BP2 | 5’-GCCCATGGTTACGCTGATTGACAATCGG-3’, Nco I, PCR for aroB |
TP1 | 5’-GCGCATGCAGGAGG TAATAAATATGTCCTCACG TAAAGAGCT-3’, Sph I, PCR for tktA |
TP2 | 5’-CGGAGCTCTTACAGCAGTTCTTTTGCTTTCG-3’, Sac I, PCR for tktA |
PntF | 5’-CAGGGTACCTCATCAATAAAACCG-3’, Kpn I, PCR for pntAB |
PntR | 5’-CGTCTGCAGTTACAGAGCTTTCAG-3’, Pst I, PCR for pntAB |
SthF | 5’-TTTTGGTACCCAGGTAAGCCCTACCATGC-3’, Kpn I, PCR for sthA |
SthR | 5’-GGGCTGCAGGGCCATTTCGATAAAGTTTT-3’, Pst I, PCR for sthA |
NadF | 5’-GCGGGGTACCATGAATAATCATTTCAAGTG-3’, Kpn I, PCR for nadK |
NAdR | 5’-GCGGTCTAGATTAGAATAATTTTTTTGACCA-3’, Xba I, PCR for nadK |
5TacF | 5’-ACGCGTGTAAAACGACGGCCAGT-3’, Mlu I, PCR for the 5Ptac promoter |
5TacR | 5'-CCGCGCATGCGGATCCGAATTCATGCATCTAGTATTTCTCCTCTTTAATGGAT-3’, Sph I, PCR for the 5Ptac promoter |
CsrF | 5’-CGGAGCTCAGGAGGTAATAAATGAGTCAGACAACGAAGTGAACAT-3’, Sac I, PCR for the csrB and pps gene cluster |
PpsR | 5’-CGGGTACCTTATTTCTTCAGTTCAGCCAGG-3’, Kpn I, PCR for the csrB and pps gene cluster |
QEF | 5’-GGATCGCCGGAATATCACCAC-3’, qPCR for the aroE gene |
QER | 5’-ACTACTGCCACTCCTTTCCCT-3’, qPCR for the aroE gene |
QBF | 5’-AAC GAA ACC CTG GCT CCT CTG-3’, qPCR for the aroB gene |
QBR | 5’-AAGCGCCACCAGCGTAGTATC-3’, qPCR for the aroB gene |
QGF | 5’-CGTTGCTGAAGTGAAAGAAGGG-3’, qPCR for the aroG gene |
QGR | 5’-ACGTCAGCACAAACATCCATC-3’, qPCR for the aroG gene |
QTF | 5’-TTTCGCCTGGCCTGCTTCTTT-3’, qPCR for the tktA gene |
QTR | 5’-CGACGCTGAAATTGCCCTGAC-3’, qPCR for the tktA gene |
QNF | 5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakK gene |
QNR | 5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakK gene |
QPF | 5’-AGCCGGAGTACGAGTTCAGCA-3’, qPCR for the pntAB gene |
QPR | 5’-ATTGCGCTGGTATTCGGCTGG-3’, qPCR for the pntAB gene |
QPPF | 5’-GACATCTTCTCGCTGACCAAC-3’, qPCR for the ppsA g ene |
QPPR | 5’-TTACCGGTGTGGCCATCTTTC-3’, qPCR for the ppsA gene |
QCSF | 5’-CTGGATGAAGCGAAGAGGATG-3’ , qPCR for the csrB gene |
QCSR | 5’-ATTGCTTCCTGCTCACACCAC-3’, qPCR for the csrB gene |
QCF | 5’-TTGTCGGCGGTGGTGATGTC-3’, qPCR for the cysG gene |
QCR | 5’-ATGCGGTGAACTGTGGAATAAACG-3’, qPCR for the cysG gene |