Table 1 Plasmids and strains used in this study

Plasmids

pACYCDuet-1

P15A origin; Cm^R; P_T7

Novagen

pTrcHis2B

ColE1 origin; Amp^R; P_trc

Invitrogen

pGH

pUC origin; Amp^R; P_T7

Generay

pTrcLower

ColE1 origin; Amp^R; P_trc:: ERG12-ERG8-ERG19-IDI1

[42]

pHB1

P15A origin; Cm^R; P_T7:: SabS1

This work

pHB2

P15A origin; Cm^R; P_T7::IspA

This work

pHB3

P15A origin; Cm^R; P_T7::IspA-SabS1

This work

pHB4

P15A origin; Cm^R; P_T7:: GPPS2

This work

pHB5

P15A origin; Cm^R; P_T7:: GPPS2-SabS1

This work

pHB6

P15A origin; Cm^R; P_T7:: mvaE-GPPS2-SabS1

pHB7

P15A origin; Cm^R; P_T7::mvaE-mvaS-GPPS2-SabS1

This work

Strains

E. coli BL21(DE3)

E. coli B dcm ompT hsdS(rB ^- mB ^-) gal

Takara

E. coli DH5α

deoR, recA1, endA1, hsdR17(rk^-, mk⁺), phoA, supE44, λ-, thi^-1, gyrA96, relA1

Invitrogen

Saccharomyces cerevisiae

Type strain

ATCC 204508

HB1

E. coli BL21(DE3) harboring pHB1

This work

HB2

E. coli BL21(DE3) harboring pHB3

This work

HB3

E. coli BL21(DE3) harboring pHB5

This work

HB4

E. coli BL21(DE3) harboring pHB7 and pTrcLower

This work