Strain or plasmid | Description | Reference |
---|---|---|
Strains | Â | Â |
S. coelicolor | Â | Â |
 M145 | Parental strain, SCP1- SCP2- | [46] |
 SC_1102 | SCO1102::Tn5062, derivative of M145; AmR | This study |
 SC_1753 | SCO1753::Tn5062, derivative of M145; AmR | This study |
 SC_1153 | SCO1102::Tn5062 SCO1753::Tn5066, derivative of SC_1102; AmR HygR | This study |
 SC_285 | M145 attB ΦBT1 ::pTR285; KmR | This study |
 SC_Lppα | M145 attB ΦBT1 ::p285-LPPα; KmR | This study |
 SC_Lppβ | M145 attBΦBT 1::p285-LPPβ; KmR | This study |
 SC_P1102 | SC_1153 attB ΦBT1 ::pRT802-LPPα; AmR HygR KmR | This study |
 SC_P1753 | SC_1153 attBΦBT 1::pRT802-LPPβ; AmR HygR KmR | This study |
E. coli | Â | Â |
 DH5α | E. coli K12 F - lacU169 (Φ80lacZΔM15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1 | [48] |
 BL21 (DE3) | F – ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3) | Novagen |
 C41 (DE3) | F – ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3) | Lucigen |
 ET 12567 | supE44 hsdS20 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-1 dam-dcm-hsdM-; CmR | [49] |
S. cerevisiae | Â | Â |
 W303-1A | MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | [50] |
 GHY58 | W303-1A dpp1 _::TRP1/KanRlpp1 _::HIS3/KanRpah1 _::URA3 | [25] |
Plasmids | Â | Â |
 pET28a | Vector for expression of N terminal His-tagged proteins under the strong T7 promoter; KmR | Novagen |
 pBAD33 | Vector for recombinant protein expression under the control of the P BAD promoter; CmR | [43] |
 pCR®-BluntII-TOPO | Vector used for cloning of blunt PCR products; KmR | Invitrogen |
 pUZ8002 | RK2 derivative with defective oriT; KmR | [49] |
 pKOS111-47 | RK2 derivative with defective oriT; ApR | B. Julien (Personal communication) |
 pQM5066 | Plasmid carrying a copy of Tn5066; HygR | P. Dyson, (Personal communication) |
 pRT802 | Intregrative vector based on ΦBT1 phage integrase; KmR | [51] |
 p425GPD | Multicopy Yeast/E. coli expression vector with GPD promoter and LEU2 marker; ApR | [45] |
 pTR285 | pRT802 derivative carrying the P ermE* promoter with no gene under its control; KmR | [52] |
 pBAD0958 | pBAD33 carrying the SCO0958His gene under the control of P BAD promoter; CmR | [52] |
 pBAD-LPPα | pBAD33 carrying the SCO1102His gene under the control of P BAD promoter; CmR | This study |
 pBAD-LPPβ | pBAD33 carrying the SCO1753His gene under the control of P BAD promoter; CmR | This study |
 pBAD-LPPγ | pBAD33 carrying the SCO6355His gene under the control of P BAD promoter; CmR | This study |
 p425-LPPα | p425-GPD carrying the SCO1102His gene under the control of GPD promoter; ApR, LEU2 | This study |
 p425-LPPβ | p425-GPD carrying the SCO1753His gene under the control of GPD promoter; ApR, LEU2 | This study |
 p425-LPPγ | p425-GPD carrying the SCO6355His gene under the control of GPD promoter; ApR, LEU2 | This study |
 p28-LPPα | pET28 carrying the SCO1102His gene under the control of T7 promoter; KmR | This study |
 p28-LPPβ | pET28 carrying the SCO1753His gene under the control of T7 promoter; KmR | This study |
 p285-LPPα | pTR285 carrying the SCO1102His gene under the control of P ermE* promoter; KmR | This study |
 p285-LPPβ | pTR285 carrying the SCO1753His gene under the control of P ermE* promoter; KmR | This study |
 pRT802-LPPα | pRT802 carrying the SCO1102His gene under the control of its own promoter; KmR | This study |
 pRT802-LPPβ | pRT802 carrying the SCO1753His gene under the control of its own promoter; KmR | This study |