Figure 6From: Identification and physiological characterization of phosphatidic acid phosphatase enzymes involved in triacylglycerol biosynthesis in Streptomyces coelicolorReconstitution of S. coelicolor de novo TAG biosynthesis pathway in E. coli . Total lipid extracts from [14C] acetic acid-labeled cultures of the indicated E. coli strains were analyzed on silica gel TLC plates and developed in hexane:diethylether:acetic acid (70:30:1, v/v/v). TAG: Triacylglycerol. FA: Fatty Acid. DAG: Diacylglycerol. PL: Phospholipid.Back to article page