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Figure 4 | Microbial Cell Factories

Figure 4

From: Identification and physiological characterization of phosphatidic acid phosphatase enzymes involved in triacylglycerol biosynthesis in Streptomyces coelicolor

Figure 4

Complementation of the temperature sensitive phenotype of dpp1 lpp1 pah1 yeast knockout strain by expressing scoLPPs. W3031A: wild type strain; GHY58 (lpp1dpp1pah1): triple knockout mutant strain; GHY58/p425GPD, GHY58/scoLppγ, GHY58/scoLppβ, GHY58/scoLppα: triple knockout strain transformed with p425GPD empty vector and with p425GPD expressing scoLppγ, scoLppβ or scoLppα. 10 μl of each dilution were spotted on the plates, and followed by incubation at 30 and 37°C for two days.

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