Figure 3
Heterologous expression of scoLPPs in E. coli and analysis of their lipid profile. (A) Immunoblot analysis of E. coli soluble fractions with anti-His antibodies. (B) Total lipid extracts from [14C] acetic acid-labelled cultures of the indicated E. coli strains were analyzed on silica gel TLC plates and developed in hexane:diethylether:acetic acid (70:30:1, v/v/v). FA: Fatty Acids. DAG: Diacylglycerol. PL: Phospholipid. (+): Inducer added. (−): No inducer.