The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6

Table 3 Hydroxylation of n-octane using permeabilised cell in 60 ml amber vials

Medium	Defined	Defined	Defined	Defined	LB	LB	LB	LB
Pre-treatment	None	Acetone	None	Acetone	None	Acetone	None	Acetone
Temperature (°C)	23	23	37	37	23	23	37	37
Max specific octanol formation rate per biomass	4.17	8.47	6.59	11.11	5.26	9.10	16.09	14.97
(μmol min ^-1 g _DCW ^-1 )

NB: Cell concentration, 3 – 4 g_DCW l^-1; reaction time, 2 h at 37°C & 6 h at 23°C; initial CYP concentration, 0.20 μmol g_DCW^-1.
The cells were pre-treated with acetone (5% v,v) prior to biotransformation.

ISSN: 1475-2859