The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6

Table 2 Comparison of key bioprocess parameters obtained in this study with previous reports on 1-octanol production using E. coli and cytochrome P450, especially the CYP153 operon

	Fujii *et al.,* 2006	Baik, 2010	Bordeaux *et al.,* 2011	Koch, et al., 2009	Gudiminchi, 2012	This study^a	This study	This study^b
E. coli biocatalyst	CYP153Aci	CYP, Fdx& Fdr	CYP153A13	CYP153A6	CYP153A6	CYP153A6	CYP153A6	CYP153A6
Growth medium	L-Broth	Marine Broth	n/a	Semi-defined	LB	LB	LB	Defined medium
Bioreactor	Vial	Shaker	n.a.	Vial	Vial	Vial	Vial	Vial
Temperature (°C)	28	37	23 ± 1	25	20	20	37	20
Biomass concentration	n.a.	n.a	n.a.	3.5	11	5.4	7.4	4.1
(g L_BRM^-1)	n.a.	n.a	n.a.	3.5	11	5.4	7.4	4.1
Max octanol formed	2.3	0.45	n.a.	0.01	8.8	6.5	2.2	6.0
(g L_BRM^-1)	2.3	0.45	n.a.	0.01	8.8	6.5	2.2	6.0
Time max octanol (h)	24	6	2	1	24	48	6	120
Max volumetric rate	0.1	0.07	n.a.	0.01	0.32	0.16	0.82	0.08
(g L_BRM^-1 h^-1)	0.1	0.07	n.a.	0.01	0.32	0.16	0.82	0.08
Biocat efficiency at max octanol_l	n.a.	n.a.	0.06	0.003	0.80	1.20	0.46	1.45
(g_octanol g_DCW^-1)	n.a.	n.a.	0.06	0.003	0.80	1.20	0.46	1.45
Max specific octanol formation rate per biomass	n.a.	n.a.	3.9	0.37	4.0	4.2	16	2.40
(μmol min^-1 g_DCW^-1)	n.a.	n.a.	3.9	0.37	4.0	4.2	16	2.40
Period_{max rate} (h)	0 – 24	2 – 6	0 – 2	0 – 1	0 – 24	0 – 24	0 – 4	24 – 48

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