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Table 1 Effect of temperature conditions on growth rate, CYP153A6 expression and subsequent bioconversion of octane to 1-octanol in a 72 h reaction

From: The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6

Temperature

Maximum growth rate

[CYP]

1-Octanol

(°C)

μmax (h-1)

(μmol gDCW-1)

(g gDCW-1)

  

A

B

A

B

20

0.20

0.17

0.16

1.00

1.00

25

0.37

0.20

0.06

1.00

0.50

30

0.50

0.17

0.00

0.80

0.00

37

0.82

0.16

0.00

0.85

0.00

  1. Under condition A, the temperature was maintained as specified prior to induction, whereafter incubation was at 20°C. Under condition B, the specified temperature was maintained throughout cultivation, expression and bioconversion. DCW, dry cell weight.