Figure 7
Whole cell hydroxylation of n-octane using resting cells grown on glucose based defined medium. The biomass was harvested and re-suspended (4 gDCW l-1) in 0.2 M Sodium Phosphate buffer, pH 7.2 consisting of glucose (starting concentration of 5 g l-1). A 1 ml aliquot of this mixture was introduced into the 60 ml amber vials and 30% v/v organic phase consisting of 200 μl octane and 100 μl BEHP was added. In another set, only octane (300 μl) was added. The vials were opened intermittently for glucose addition and also to allow inlet of air to prevent oxygen limitation. The reaction was mixed on an orbital shaker at 200 rpm and 20°C.