Figure 6
(A) Whole cell hydroxylation of n– octane using biomass from LB cultures with in situ product removal using bis (2- ethylhexyl) phthalate. The bio-reaction mixture consisted of 1 ml re-suspended cells (5 gDCW l-1) in 0.2 M sodium phosphate buffer, pH 7.2, and 30% v/v organic phase consisting of 100 μl BEHP. (B) Glucose concentration was monitored in each of the systems (with and without co-solvent) as well as a control system (without organic phase). The vials were opened intermittently for glucose addition and also to allow air inlet to prevent oxygen limitation. The reaction was mixed on an orbital shaker at 200 rpm and 20°C.