From: Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli
Strain | Glycerol consumed (g/L) | Product synthesized (g/L) | Carbon recoveryb | ||||||
---|---|---|---|---|---|---|---|---|---|
Acetate | Succinate | Ethanol | Lactate | Pyruvate | Biomass | Products | Overall | ||
Wild-type strain | |||||||||
MG1655 | 19.1 (0.1) | 2.9 (0.1) | 2.4 (0.0) | 2.1 (0.1) | 1.6 (0.3) | 0.0 (0.0) | 1.2 (0.0) | 67.4 (1.4) | 75.4 (1.6) |
Strains engineered for the production of D-lactate | |||||||||
LA01 | 18.2 (1.1) | 0.6 (0.1) | 0.0 (0.0) | 0.0 (0.0) | 12.3 (0.6) | 0.0 (0.0) | 1.4 (0.0) | 74.9 (3.4) | 84.3 (3.7) |
LA02 | 15.6 (1.8) | 0.6 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 10.9 (1.0) | 0.0 (0.0) | 1.2 (0.1) | 77.6 (2.8) | 87.4 (3.4) |
Strains engineered for the production of L-lactate | |||||||||
LA06 | 11.0 (0.2) | 2.0 (0.1) | 0.1 (0.0) | 0.0 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 1.2 (0.0) | 27.8 (0.5) | 67.2 (0.9) |
LA07 | 9.8 (0.7) | 1.2 (0.4) | 0.2 (0.1) | 0.0 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 0.35 (0.1) | 20.7 (5.5) | 34.0 (2.2) |
LA06 (pZS) | 9.8 (0.1) | 1.8 (0.1) | 0.1 (0.0) | 0.0 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 1.1 (0.1) | 29.2 (1.6) | 70.0 (0.1) |
LA07 (pZS) | 9.9 (0.2) | 1.5 (0.0) | 0.3 (0.0) | 0.0 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 0.4 (0.0) | 30.1 (0.6) | 46.7 (2.0) |
LA06 (pZSldh) | 11.8 (0.1) | 0.6 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 6.4 (0.0) | 0.0 (0.0) | 1.3 (0.0) | 64.9 (0.2) | 78.6 (0.2) |
LA07 (pZSldh) | 10.9 (0.5) | 0.2 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 6.7 (0.4) | 0.0 (0.0) | 0.3 (0.1) | 65.7 (2.9) | 70.4 (1.9) |
LA19 | 16.2 (0.2) | 1.0 (0.0) | 0.0 (0.0) | 0.0 (0.0) | 9.1 (0.1) | 0.0 (0.0) | 0.9 (0.1) | 67.4 (0.2) | 74.5 (1.1) |
LA20 | 15.4 (0.1) | 0.4 (0.0) | 0.2 (0.0) | 0.0 (0.0) | 9.4 (0.1) | 0.0 (0.0) | 1.0 (0.1) | 67.7 (0.2) | 75.8 (0.5) |
LA19 (pZSKLMgldA) | 16.6 (0.6) | 1.0 (0.0) | 0.0 (0.0) | 0.2 (0.0) | 10.9 (0.4) | 0.0 (0.0) | 0.4 (0.0) | 79.0 (0.4) | 82.1 (0.9) |
LA19 (pZSglpKglpD) | 16.8 (0.9) | 0.7 (0.1) | 0.0 (0.0) | 0.0 (0.0) | 11.7 (1.2) | 0.0 (0.0) | 0.8 (0.0) | 79.7 (3.9) | 85.9 (3.8) |
LA19 (pZSldh) | 13.2 (1.6) | 0.6 (0.1) | 0.0 (0.0) | 0.0 (0.0) | 7.4 (1.3) | 0.0 (0.0) | 0.7 (0.1) | 65.6 (4.6) | 74.4 (3.4) |
LA20 (pZSKLMgldA) | 15.2 (0.3) | 0.4 (0.1) | 0.1 (0.0) | 0.0 (0.0) | 10.1 (0.5) | 0.0 (0.0) | 0.9 (0.2) | 72.9 (1.5) | 80.3 (3.1) |
LA20 (pZSglpKglpD) | 18.6 (0.4) | 0.6 (0.0) | 0.2 (0.0) | 0.0 (0.0) | 13.7 (0.7) | 0.0 (0.0) | 0.8 (0.0) | 76.4 (1.7) | 82.8 (1.6) |
LA20 (pZSldh) | 15.3 (0.2) | 0.2 (0.0) | 0.2 (0.1) | 0.0 (0.0) | 10.0 (0.1) | 0.0 (0.0) | 0.9 (0.0) | 70.5 (1.6) | 77.6 (2.0) |
LA20 (pZSglpK.glpD)c | 41.0 (0.0) | 1.0 (0.0) | 0.7 (0.0) | 0.0 (0.0) | 32.6 (0.1) | 0.0 (0.0) | 1.1 (0.0) | 86.4 (0.3) | 89.6 (0.3) |
LA20ΔlldD (pZSglpKglpD)c | 41.6 (0.0) | 0.8 (0.0) | 0.6 (0.0) | 0.0 (0.0) | 34.7 (0.0) | 0.0 (0.0) | 1.6 (0.0) | 90.3 (0.0) | 95.0 (0.1) |
LA20ΔlldD (pZSglpKglpD)d | 40.4 (0.0) | 0.9 (0.0) | 0.9 (0.0) | 0.0 (0.0) | 32.8 (0.1) | 0.0 (0.0) | 1.1 (0.0) | 88.4 (0.2) | 91.9 (0.1) |
LA20ΔlldD pZSglpKglpD)e | 57.2 (0.0) | 1.6 (0.0) | 1.2 (0.0) | 0.0 (0.0) | 50.1 (0.0) | 0.0 (0.0) | 2.0 (0.0) | 95.2 (0.1) | 99.4 (0.7) |