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Table 2 Specific activity measured from the crude lysate of the engineered β-glucosidase

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

 

p-NGP

Cellobiose

 

(U/mg protein)

(U/mg protein)

Control

0.00729 ± 0.0001

0.0021 ± 0.002

Wild-type

0.193 ± 0.01

0.0668 ± 0.001

L173H

0.130 ± 0.01

0.124 ± 0.004

H23L

0.219 ± 0.004

0.0738 ± 0.006

L173H + H23L

0.156 ± 0.007

0.170 ± 0.01

  1. One unit is defined as one μmol/min and the control is the lysate from a strain with no heterologously expressed β-glucosidase. Errors are derived from the standard deviation of biological triplicates.
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Microbial Cell Factories

ISSN: 1475-2859

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