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Figure 5 | Microbial Cell Factories

Figure 5

From: Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Figure 5

Sucrose utilisation and GFP activity in strain MGΔ lacZ :: xyn Δ rbsAR :: gfp Δ arsB :: csc . A) MacConkey agar supplemented with 1% sucrose was used to examine sucrose fermentation in wild type MG1655 (left) and engineered strain MGΔlacZ::xyn ΔrbsAR::gfp ΔarsB::csc. The ability to utilise sucrose is indicated by a pH change resulting from fermentation products; this is evidenced by red colonies on the MacConkey sucrose plate. B) Wild type MG1655 exhibits no fluorescence in the presence of UV light (left), whereas the engineered strain MGΔlacZ::xyn ΔrbsAR::gfp ΔarsB::csc fluoresces bright green (right).

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Microbial Cell Factories

ISSN: 1475-2859

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