Table 2 Primers used for cloning of the expression vectors

P _MET _fw (BstX I):

TAGACCAAGGCCTTGG AATTCAGGCAACAGGACC

P _MET _rv (Sbf I):

AATACCTGCAGG TTCTTTCTGAGTTGGTTTCC

P _THR _fw (BstX I):

TAGACCAAGGCCTTGG TTCTTCAGAATGGGAACTAG

P _THR _rv (Sbf I):

AATACCTGCAGG TGTCTGCCACACAAATTAA

P _SER _fw (BstX I):

TAGACCAAGGCCTTGG CAGCAAATAATTAGCAGCC

P _SER _rv (Sbf I):

AATACCTGCAGG TGTATTATATGGTTAGTTCAAGATG

P _PIS _fw (BstX I):

TAGACCAAGGCCTTGG GTAACGAGGCTAAAAGTTTTTGC

P _PIS _rv (Sbf I):

AATACCTGCAGG TGCAGGTGGACTATCTAGAGACAAG

P _THI _fw (BstX I):

TAGACCAAGGCCTTGG CATCTTTTCAGCTTCATCGTCAG

P _THI _rv (Sbf I):

AATACCTGCAGG ATGATTTATTGAAGTTTCCAAAGTTG

P _ERO _fw (Asc I):

ATTAGGCGCGCC AAGGGAACCCATTTTCTTCG

P _ERO _rv (Apa I):

ATTAGGGCCC GGTAGTGGAACAGCAAGATGG

P _PDI _fw (Asc I):

ATTAGGCGCGCC ATTCCGGAGATTCACATTGC

P _PDI _rv (Apa I):

ATTAGGGCCC TCATCGGGCAGTTCTTTCTT