Skip to main content

Table 2 Intracellular concentrations of NAD + , NADH, NADP + and NADPH during exponential growth in batch cultures

From: Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement

Strains

Intracellular concentration (μmol/g biomass)

NADH/ NAD+ratio

NADPH/ NADP+ratio

 

NAD+

NADH

NADP+

NADPH

  

D852

2.33 ± 0.05

0.85 ± 0.03

0.72 ± 0.04

1.89 ± 0.05

0.36 ± 0.03

2.63 ± 0.22

HT-ΔGDH

1.31 ± 0.05

0.59 ± 0.10

0.65 ± 0.06

3.31 ± 0.09

0.45 ± 0.10

5.09 ± 0.67

HT-ΔPPC

2.67 ± 0.02

0.73 ± 0.06

0.95 ± 0.06

1.38 ± 0.07

0.27 ± 0.03

1.45 ± 0.18

HT-DAHP

2.58 ± 0.02

0.88 ± 0.05

0.68 ± 0.07

1.80 ± 0.02

0.34 ± 0.02

2.65 ± 0.33

HT-PNT

2.55 ± 0.09

0.59 ± 0.04

0.47 ± 0.03

2.19 ± 0.06

0.23 ± 0.03

4.66 ± 0.45

HT-ACC

2.47 ± 0.04

0.95 ± 0.02

0.67 ± 0.07

1.68 ± 0.04

0.38 ± 0.02

2.50 ± 0.37

HT-ZWF

2.35 ± 0.09

0.91 ± 0.04

0.34 ± 0.03

2.28 ± 0.06

0.39 ± 0.03

6.71 ± 0.84

  1. Results are represented as mean ± SD of three independent observations.