Table 1 Specific activity of enzymes by parent strain S. tsukubaensis D852 and recombinants in batch cultures

GDH1

72

55.85 ± 1.36

0.25 ± 0.05

-

-

-

-

-

144

36.47 ± 1.09

0.13 ± 0.04

-

-

-

-

-

GDH2

72

60.75 ± 5.21

79.55 ± 1.66

-

-

-

-

-

144

50.32 ± 3.98

54.67 ± 4.42

-

-

-

-

-

PPC

72

0.85 ± 0.04

-

0.08 ± 0.05

-

-

-

-

144

0.32 ± 0.11

-

0.10 ± 0.04

-

-

-

-

PK

72

0.86 ± 0.02

-

1.12 ± 0.03

-

-

-

-

144

0.47 ± 0.03

-

0.60 ± 0.02

-

-

-

-

PC

72

2.25 ± 0.22

-

2.49 ± 0.16

-

-

-

-

144

1.77 ± 0.13

-

1.89 ± 0.12

-

-

-

-

DAHP

72

0.15 ± 0.03

-

-

0.26 ± 0.02

-

-

-

144

0.11 ± 0.03

-

-

0.19 ± 0.04

-

-

-

PNT

72

0.07 ± 0.02

-

-

-

0.98 ± 0.02

-

-

144

0.19 ± 0.05

-

-

-

1.69 ± 0.09

-

-

ACC

72

Not detected

-

-

-

-

Not detected

-

144

Not detected

-

-

-

-

Not detected

-

G6PDH

72

0.13 ± 0.03

-

-

-

-

-

0.29 ± 0.04

144

0.08 ± 0.02

-

-

-

-

-

0.15 ± 0.05