Figure 5

Intracellular metabolite profiles at 72 h for engineered strains. Heat map visualizes most intracellular metabolites from glycolysis, TCA, PPP and amino acid pathways of engineered strains under the same fermentation condition. The color code indicates an increased (red) or a decreased (green) availability as compared to the reference wild-type strain D852, as indicated by the color legend as aside the graph. Availability for each metabolite was calculated as ratio of the concentration of each engineered strain to that of the wild-type strain D852. The data are the average values of at least five series of five parallel tests.