Figure 3
Single gene knockout, overexpression and knockout-overexpression combinations target identification in S. tsukubaensis . (A) The effect of single gene knockout on the specific FK506 production rate and the specific growth rate. (B) The effect of single gene overexpression on the specific FK506 production and fPH. (C) The effect of knockout-overexpression combinations on the specific FK506 production and fPH. The enzymes encoded by these genes are as follows: aceA, isocitrate lyase; aceB2/B1, malate synthase; sucC/D, succinyl-CoA synthase; gdhA, glutamate dehydrogenase; pgi, glucose-6-phosphate isomerase; ppc, phosphoenolpyruvate carboxylase; dahp, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase; pntAB, pyridine nucleotide transhydrogenase; accA2, acetyl-CoA carboxylase; fkbA, fkbB, fkbC, polyketide synthase; fkbO, chorismatase, 4,5-dihydroxycyclohex-1-enecarboxylic acid (DHCHC) synthesis; fkbL, lysine cyclodeaminase; fkbP, non-ribosomal peptide synthetase; fkbM, 31-O-methyltrasferase; fkbD, C9 hydroxylase; fkbG, fkbH, fkbI, fkbJ, fkbK, genes for methoxymalonyl-ACP synthesis; tcsA, tcsB, tcsC, tcsD, genes for allymalonyl-CoA synthesis; zwf2, glucose-6-phosphate dehydrogenase; pccB, propionyl-CoA carboxylase; metK, S-adenosylmethionine synthetase.